Samant Lab Publications

(* = equal contribution; ^@ = corresponding author(s))

Pre-prints (under review)

Ubi-SCAPE enables deep exploration of the poly-ubiquitylome.

Johnston HE^@, Frey A, Barve G, Carling S, Trost M, Samant RS^@. bioRxiv  10.64898/2026.02.13.703496 | PRIDE dataset

Declining intracellular proteostasis capacity drives misfolded protein secretion in senescent human cells.

Watts T, Johnston HE, Al-Mufti Y, Odle RI, Hodgson RE, Okkenhaug H, Carr H, Cranwell S, Wu E, Walker S, Andrews S, Shelkovnikova T, Samant RS^@. bioRxiv  10.1101/2025.09.07.674107 | PRIDE: dataset 1 | PRIDE: dataset 2 | PRIDE: dataset 3

Peer-reviewed articles

Native size-exclusion chromatography–based mass spectrometry (SEC-MS) reveals new components of the early Heat Shock Protein 90 inhibition response among limited global changes.

Samant RS*^@, Batista SA*, Larance M, Ozer B, Milton CI, Bludau I, Wu E, Biggins L, Andrews S, Hervieu A, Johnston HE, Al-Lazikhani B, Lamond AI, Clarke PA, Workman P^@. Mol Cell Proteomics 2023; 22: 100485
 10.1016/j.mcpro.2022.100485 | PubMed | pre-print | web-resource | PRIDE dataset|

Solvent precipitation SP3 (SP4) enhances recovery for proteomics sample preparation without magnetic beads.

Johnston HE^@, Yadav K, Kirkpatrick JM, Biggs GS, Oxley D, Kramer HB, Samant RS^@. Anal Chem 2022; 94: 10320–8
 10.1021/acs.analchem.1c04200 |  PubMed |  pre-print |  PRIDE: dataset 1 |  PRIDE: dataset 2 |  PRIDE: dataset 3 |  GenEngNews|

Reviews

Ubiquitylomics: an emerging approach for profiling protein ubiquitylation in skeletal muscle.

Lord SO, Johnston HE, Samant RS, Lai YC^@. J Cachexia Sarcopenia Muscle 2024; 15: 2281–94
 10.1002/jcsm.13601 |  PubMed|

Alternative systems for misfolded protein clearance: life beyond the proteasome.

Johnston HE, Samant RS^@. FEBS J 2020; 15: 4464–87
 10.1111/febs.15617 |  PubMed|

Book chapters

Introduction: Molecular Chaperones and Protein Quality Control.

Balchin D^@, Aguilar Rangel M, Samant RS^@. Biophysics of Molecular Chaperones 2023; Chapter 1: 1–37
 10.1016/B978-0-323-91617-2.00004-3 |

Dysregulated proteostasis: mechanisms and links to aging.

Al-Mufti Y*, Cranwell S*, Samant RS^@. Molecular, Cellular, and Metabolic Fundamentals of Human Aging 2022; Chapter 4: 55–103
 10.1039/BK9781839165986-00001 |

Rahul’s earlier work

Mediator kinase inhibitor selectivity and activity in colorectal cancer.

ACS Chemical Biology 2025; 20: 1792–804  10.1021/acschembio.5c00338 |  PubMed |

A hierarchical assembly pathway directs the unique subunit arrangement of TRiC/CCT.

Mol Cell 2023; 20: 1792–804  10.1016/j.molcel.2023.07.031|  PubMed |

Native mass spectrometry analyses of chaperonin complex TRiC/CCT reveal subunit N-terminal processing and re-association patterns.

Sci Rep 2021; 11: 13084  10.1038/s41598-021-91086-6 |  PubMed |

Dosage compensation plans: protein aggregation provides additional insurance against aneuploidy.

Genes Dev 2019; 33: 1027–30
 10.1101/gad.329383.119 | PubMed |

Methods for measuring misfolded protein clearance in the budding yeast Saccharomyces cerevisiae.

Methods Enzymol 2019; 619: 27–45
 10.1016/bs.mie.2018.12.039 | PubMed |

Distinct proteostasis circuits cooperate in nuclear and cytoplasmic protein quality control.

Nature 2018; 563: 407–11
10.1038/s41586-018-0678-x | PubMed | PRIDE dataset |

Mechanisms and functions of spatial protein quality control.

Annu Rev Biochem 2017; 86: 33.1–33.26
 10.1146/annurev-biochem-060815-014616 | PubMed |

A selective chemical probe for exploring the role of CDK8 and CDK19 in human disease.

Nat Chem Biol 2015; 11: 973–80
 10.1038/nchembio.1952 | PubMed | ScienceDaily | EurekaAlert | MedicalExpress | CardiffUniversity | icr 2016 highlights |

E3 ubiquitin ligase CUL5 modulates multiple molecular and cellular responses to HSP90 inhibition in human colon cancer cells.

Proc Natl Acad Sci USA 2014; 111: 6834–9
 10.1073/pnas.1322412111 | PubMed | ScienceDaily | EurekaAlert | MedicalExpress | PennyWarren | HuffPost | icr 2014 highlights |

ATP-competitive inhibitors block protein kinase recruitment to the Hsp90-Cdc37 system.

Nat Chem Biol 2013; 9: 307–12
 10.1038/nchembio.1212 |  PubMed |  commentary |  MedicalNewsToday |  icr 2013 highlights |

Molecular biology: choose your protein partners.

Nature 2012; 490: 351–2
 10.1038/490351a |  PubMed |

The expanding proteome of HSP90.

Cell Cycle 2012; 11: 1301–12
10.4161/cc.19722 | PubMed |